Strategies in megasynthase engineering – fatty acid synthases (FAS) as model proteins

• Manuel Fischer and
• Martin Grininger

Beilstein J. Org. Chem. 2017, 13, 1204–1211, doi:10.3762/bjoc.13.119

• ; protein design; Review Megasynthases are proteins in natural compound synthesis Microbial natural products represent a rich source of pharmaceutically relevant chemical entities. A major class is represented by polyketides (PK) exemplified by the antibiotics erythromycin and rifamycin, by the

Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

• Michaela Mühlberg,
• Michael G. Hoesl,
• Christian Kuehne,
• Jens Dernedde,
• Nediljko Budisa and
• Christian P. R. Hackenberger

Beilstein J. Org. Chem. 2015, 11, 784–791, doi:10.3762/bjoc.11.88

• . Results and Discussion Protein design Aha labelled TTL variants were always expressed with the SPI approach. Aha is a Met analogue and incorporation leads to full substitution of all Met residues in TTL by Aha residues. Six of the ten Met positions are solvent accessible (M1,M20, M21, M145, M150, M161

• -terminus leaving the TEV protease recognition site inaccessible for the protease (for more information on protein design see Supporting Information File 1). Therefore, the construct was altered to contain an unmodified N-terminus with Ser at position 2. The N-terminal Met is cleaved when followed by small

• (Coomassie stain), C) Western Blot (streptavidin-peroxidase antibody). SPR measurements: A) set-up showing different binding events of the double-functionalized TTL to ECL; B) KD-data obtained for binding of Gal-0, Gal-1 and Gal-3. Protein design and dual-functionalization of TTL: periodate cleavage, oxime

Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique

• Raheleh Rezaei Araghi,
• Carsten C. Mahrenholz,
• Rudolf Volkmer and
• Beate Koksch

Beilstein J. Org. Chem. 2012, 8, 640–649, doi:10.3762/bjoc.8.71

• pathways [1]. Coiled coils have several advantageous features that, on the one hand, allow them to fulfill a wide range of important cellular functions [2] and, on the other hand, make them ideal building blocks in protein design: They are ubiquitous proteins that have the ability to oligomerize with high

Coupled chemo(enzymatic) reactions in continuous flow

• Ruslan Yuryev,
• Simon Strompen and
• Andreas Liese

Beilstein J. Org. Chem. 2011, 7, 1449–1467, doi:10.3762/bjoc.7.169

• , or “fitting” them to a particular operation window by means of directed evolution or protein design [58], is a good way to make continuous coupled-reaction (chemo)enzymatic processes more appealing for practical purposes. And proceeding in this way would represent the realization of one more